Reference
Cross-species Testing & L-Series Arrays
Cross-species Testing
Customers often ask about using Raybiotech antibody arrays with species other than human, mouse or rat. In this article, we address this question.
Cross-Species Testing with Sandwich Arrays
Sandwich ELISA arrays include our classic membrane arrays and glass-slide arrays. This means they use antibody pairs to detect proteins, just like a regular ELISA, only multiplexed to detect multiple proteins at once.
Because these arrays use ELISA antibody pairs, just like single ELISAs, they are highly specific with limited cross-species reactivity.
There are some exceptions. While these examples will not guarantee your samples will work as well, it is evidence that they might.
Not surprisingly, several papers have published using our sandwich ELISA arrays with non-human primate samples, including one using a Quantibody array with samples from Mandrills:
Soquiere, et al. J Med Primatol, 2009. Others include Chimpanzee samples (
Kim, et al. J Virol, 2007), and Green Monkey COS-7 cell culture (
Piwnica, et al. Mol Endocrinol, 2004);
Three papers have been published using our human sandwich ELISA arrays with pig (porcine) samples:
Hausman, et al. J Animal Sci, 2006;
Pisarlu, et al. J Am Coll Cardiol, 2006.;
Kuusneimi, et al. Kidney Intl, 2005.
A number of papers have been published using our human sandwich ELISA arrays with rabbit (lapine) samples:
He, et al. Stroke, 2004;
Ribichini, et al. J Am Coll Cardiol, 2007;
Joner, et al. Arterioscler Thromb Vasc Biol, 2007;
Yukihiro, et al. Spine, 2004;
John, et al. J Am Coll Cardiol Intv, 2008.
And one publication uses guinea pig samples with our human sandwich ELISA arrays:
Swifka, et al. Cardiovasc Drugs Ther, 2008.
Cross-species Testing with Label-based ArraysOur Label-based Arrays offer even greater opportunities for cross-species reactivity.
When we tested 100% bovine serum on our human sandwich ELISA arrays, we got almost no response. However, if you run media containing just 2% fetal bovine serum (FBS) on our human Label-based array, you get a discernible expression pattern. Thus, if you are running cell-cultured media containing FBS, you should run a media-only blank as a control.
However, if you have an sample from a species such as ferret, cat, or dog, your chances of finding an array to detect these proteins are small, unless you use our Label-based arrays. In fact, we have tested such samples in our lab, with good results: ferret samples with the Mouse L-Series 308, and feline and canine samples with our Human L-Series 508.
As you can imagine, if these disparate samples work, then the cross-reactivity with non-human primate samples is even better. Below is a side-by-side comparison of human (left) and rhesus monkey (right) serum samples run on L-Series 507 membranes. You can see that the expression patterns are almost identical.
So, if you want to use our arrays with samples other than those from human, mouse, or rat, your best bet is to use one of our Label-based arrays.
Human Label-Based Arrays (detects 507 proteins)
Human L-507 Antibody ListMembrane Kit Manual (AAH-BLM-1)Glass-slide Kit Manual (AAH-BLG-1)Human Label-Based Adipokine Arrays (detects 182 proteins)
Human Adipokine Array Antibody ListMembrane Kit Manual (AAH-BLM-ADI-1)Glass-slide Kit Manual (AAH-BLG-ADI-1)Mouse Label-Based Arrays (detects 308 proteins)
Mouse L-308 Antibody ListMembrane Kit Manual (AAM-BLM-1)Glass-slide Kit Manual (AAM-BLG-1)Rat Label-Based Arrays (detects 90 human proteins)
Rat L-90 Antibody ListMembrane Kit Manual (AAR-BLM-1)Glass-slide Kit Manual (AAR-BLG-1)
