Antibody pair for Duolink in situ PLA

Antibody Pair for in situ PLA™





A powerful platform for protein phosphorylation and protein-protein interaction studies in situ is created by combining Abnova's dual recognition, primary antibody pair with Duolink® in situ proximity ligation assay (in situ PLA™) from Olink Bioscience. This revolutionary combination enables unprecedented specificity and sensitivity of protein detection and quantification for high performance immunofluorescence and immunohistochemistry applications. Leveraging on Abnova's manufacturing capability and growing collection of 30,000+ antibodies, and Olink Bioscience in situ PLA technology, hundreds of target specific and validated assays are being developed and marketed by both companies in cells and tissue samples.

Principle of Duolink


1. Incubate with target primary antibodies from two different species


2. Add PLA probes PLUS and MINUS


3. Hybridize connector oligos


4. Ligation to form a complete DNA circle


5. Rolling circle amplification


6. Add fluorescent probes to reveal phosphorylation

Antibody pairs for Duolink in situ PLA

Catalog # Product Name
DP0052Phospho-BRAF(S445) & BRAF Dual Recognition Antibody Pair
DP0042 Phospho-CDK2(T160) & CDK2 Dual Recognition Antibody Pair
DP0022 Phospho-CDKN1A(T145) & CDKN1A Dual Recognition Antibody Pair
DP0024 Phospho-CDKN1A(T57) & CDKN1A Dual Recognition Antibody Pair
DP0017 Phospho-DNMT1(S714) & DNMT1 Dual Recognition Antibody Pair
DP0002 Phospho-DNMT1(Y399) & DNMT1 Dual Recognition Antibody Pair
DP0016 Phospho-DNMT1(Y969) & DNMT1 Dual Recognition Antibody Pair
DP0020 Phospho-EGFR(S1166) & EGFR Dual Recognition Antibody Pair
DP0027 Phospho-EGFR(Y1197) & EGFR Dual Recognition Antibody Pair
DP0034 Phospho-FGFR1(Y154) & FGFR1 Dual Recognition Antibody Pair
DP0025 Phospho-FGFR1(Y463) & FGFR1 Dual Recognition Antibody Pair
DP0043 Phospho-IKBKB(Y199) & IKBKB Dual Recognition Antibody Pair
DP0040 Phospho-IKBKG(S31) & IKBKG Dual Recognition Antibody Pair
DP0035 Phospho-MAP2K5(S137) & MAP2K5 Dual Recognition Antibody Pair
DP0011 Phospho-MAP2K5(S149) & MAP2K5 Dual Recognition Antibody Pair
DP0039 Phospho-MAP3K5(S83) & MAP3K5 Dual Recognition Antibody Pair
DP0050 Phospho-MAP3K5(S966) & MAP3K5 Dual Recognition Antibody Pair
DP0037 Phospho-MAP4K4(S631) & MAP4K4 Dual Recognition Antibody Pair
DP0030 Phospho-MCAM(Y641) & MCAM Dual Recognition Antibody Pair
DP0021 Phospho-NFKB1(S337) & NFKB1 Dual Recognition Antibody Pair
DP0023 Phospho-NFKB1(S893) & NFKB1 Dual Recognition Antibody Pair
DP0036 Phospho-NFKB1(S932) & NFKB1 Dual Recognition Antibody Pair
DP0053 Phospho-PAK1(T423) & PAK1 Dual Recognition Antibody Pair
DP0010 Phospho-PDGFRA(Y1018) & PDGFRA Dual Recognition Antibody Pair
DP0013 Phospho-PDGFRA(Y572) & PDGFRA Dual Recognition Antibody Pair
DP0009 Phospho-PDGFRA(Y574) & PDGFRA Dual Recognition Antibody Pair
DP0028 Phospho-PDGFRA(Y754) & PDGFRA Dual Recognition Antibody Pair
DP0031 Phospho-PDGFRA(Y988) & PDGFRA Dual Recognition Antibody Pair
DP0006 Phospho-PDGFRB(Y1021) & PDGFRB Dual Recognition Antibody Pair
DP0004 Phospho-PDGFRB(Y579) & PDGFRB Dual Recognition Antibody Pair
DP0005 Phospho-PDGFRB(Y740) & PDGFRB Dual Recognition Antibody Pair
DP0054 Phospho-PRL(S163) & PRL Dual Recognition Antibody Pair
DP0003 Phospho-RAF1(S259) & RAF1 Dual Recognition Antibody Pair
DP0019 Phospho-RAF1(S339) & RAF1 Dual Recognition Antibody Pair
DP0029 Phospho-RAF1(Y340) & RAF1 Dual Recognition Antibody Pair
DP0012 Phospho-SMAD3(S208) & SMAD3 Dual Recognition Antibody Pair
DP0026 Phospho-SMAD3(S213) & SMAD3 Dual Recognition Antibody Pair
DP0015 Phospho-TP53(S37) & TP53 Dual Recognition Antibody Pair
DP0014 Phospho-TP53(S9) & TP53 Dual Recognition Antibody Pair

Duolink reagents

Catalog # Product Name
Art no. 90202 Duolink PLA probe Anti-Rabbit MINUS
Art no. 90301 Duolink PLA probe Anti-Mouse PLUS
Art no. 90103 Duolink 100 Detection Kit 613
or Art no. 90104 Duolink 100 Detection Kit 563
Art no. 80100 Duolink Mounting Medium, 10ml