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Vector DyLight
DyLight Conjugates
DyLight® fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. In comparison, the DyLight ® dyes offer a number of potential advantages including greater photostability and brighter fluorescence. Vector Labs have introduced these enhanced fluorescent compounds conjugated to affinity-purified secondary antibodies and streptavidin for use in a variety of applications in particular cell and tissue based immunofluorescent antigen detection/visualization.
The Dylight® dyes currently offered are DyLight® 488 (green), DyLight® 549 (orange), and DyLight® 594 (red). The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets. Furthermore, the DyLight® dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight® dyes can be applied as single labels or in combination with other DyLight® dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology.
Conjugate Excitation max.(nm) Emission max.(nm) Spectrallysimilar dyes
---------------------------------------------------------------------------------------
DyLight 488 493 518 FITC, Alexa Fluor 488, Cy2
DyLight 549 556 571 TRITC, Alexa Fluor 555, Cy3
DyLight 594 592 617 Alexa Fluor 594, Texas Red
DyLight 649 655 670 Alexa Fluor 647, Cy5
DI-2488 DyLight 488 Anti-Mouse IgG (H+L) 4.058Kč
DI-1488 DyLight 488 Anti-Rabbit IgG (H+L) 4.058Kč
SA-5488 DyLight 488 Streptavidin 4.218Kč
DI-2549 DyLight 549 Anti-Mouse IgG (H+L) 4.058Kč
DI-1549 DyLight 549 Anti-Rabbit IgG (H+L) 4.058Kč
SA-5549 DyLight 549 Streptavidin 4.218Kč
DI-2594 DyLight 594 Anti-Mouse IgG (H+L) 4.058Kč
DI-1594 DyLight 594 Anti-Rabbit IgG (H+L) 4.058Kč
SA-5594 DyLight 594 Streptavidin 4.218Kč
DI-1649 DyLight 649 Anti-Rabbit IgG (H+L) 4.058Kč
DI-2649 DyLight 649 Anti-Mouse IgG (H+L) 4.058Kč
SA-5649 DyLight 649 Streptavidin 4.218Kč
DyLight® fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. In comparison, the DyLight ® dyes offer a number of potential advantages including greater photostability and brighter fluorescence. Vector Labs have introduced these enhanced fluorescent compounds conjugated to affinity-purified secondary antibodies and streptavidin for use in a variety of applications in particular cell and tissue based immunofluorescent antigen detection/visualization.
The Dylight® dyes currently offered are DyLight® 488 (green), DyLight® 549 (orange), and DyLight® 594 (red). The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets. Furthermore, the DyLight® dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight® dyes can be applied as single labels or in combination with other DyLight® dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology.
Conjugate Excitation max.(nm) Emission max.(nm) Spectrallysimilar dyes
---------------------------------------------------------------------------------------
DyLight 488 493 518 FITC, Alexa Fluor 488, Cy2
DyLight 549 556 571 TRITC, Alexa Fluor 555, Cy3
DyLight 594 592 617 Alexa Fluor 594, Texas Red
DyLight 649 655 670 Alexa Fluor 647, Cy5
DI-2488 DyLight 488 Anti-Mouse IgG (H+L) 4.058Kč
DI-1488 DyLight 488 Anti-Rabbit IgG (H+L) 4.058Kč
SA-5488 DyLight 488 Streptavidin 4.218Kč
DI-2549 DyLight 549 Anti-Mouse IgG (H+L) 4.058Kč
DI-1549 DyLight 549 Anti-Rabbit IgG (H+L) 4.058Kč
SA-5549 DyLight 549 Streptavidin 4.218Kč
DI-2594 DyLight 594 Anti-Mouse IgG (H+L) 4.058Kč
DI-1594 DyLight 594 Anti-Rabbit IgG (H+L) 4.058Kč
SA-5594 DyLight 594 Streptavidin 4.218Kč
DI-1649 DyLight 649 Anti-Rabbit IgG (H+L) 4.058Kč
DI-2649 DyLight 649 Anti-Mouse IgG (H+L) 4.058Kč
SA-5649 DyLight 649 Streptavidin 4.218Kč