Insectomed SF express: Serum-free insect cell medium
Already tested successfully for the growth of baculovirus-infected SF21 cells.
Insectomed SF express is suitable for the cultivation of Spodoptera frugiperda (SF9, SF21), BTI-TN-5B1-4 (High Five™) cells and Drosophila melanogaster (e.g. D.Mel-2)-cells. Whether we're talking about monolayer,
spinner or shake cultures, adherent cells or suspension cells: All cell types can be cultivated therein. As a complete medium, it contains all the additives required for the cultivation of insect cells, in optimum
concentration: steroids, amino acids, organic acids, glutamine, and glucose. The complete medium is suitable for the expression of recombinant proteins.
Insectomed SF express is suitable for the cultivation of Spodoptera frugiperda (SF9, SF21), BTI-TN-5B1-4 (High Five™) cells and Drosophila melanogaster (e.g. D.Mel-2)-cells. Whether we're talking about monolayer,spinner or shake cultures, adherent cells or suspension cells: All cell types can be cultivated therein. As a complete medium, it contains all the additives required for the cultivation of insect cells, in optimumconcentration: steroids, amino acids, organic acids, glutamine, and glucose. The complete medium is suitable for the expression of recombinant proteins.
Hints for adaption of the cells you’ll find here:
http://www.biochrom.de/en/service/tips-anddetails/media/
Our customers have already tested Insectomed SF express:
„Insectomed SF express is recommended for the cultivation of insect cells and for infection with baculovirus. The cells were successfully introduced into the new medium from the preculture. The infection rate of SF21 cells was nearly 100 % after three days.“ (A. Amtmann, Institute of Bioprocess Engineering, Friedrich-Alexander University of Erlangen-Nuremberg, Germany)
All advantages at a glance:
- animal component-free
- „ready-to-use“
- no supplements
- successfully tested and recommended
Fig.: Growth curve of SF21 cultures infected with baculovirus
After 54 hours the infection rate was 94 % or 99 % relating to
the product development of GFP.
(low cell rate: 7.1x10^5 cells, high cell rate: 3.3x10^6 cells)
F 8275, Insectomed, 500ml Volejte Kč
