JetPRIME™ DNA & siRNA Transfection Reagent

JetPRIME™ DNA & siRNA Transfection Reagent




JetPrime transfection reagent can now be used for DNA and siRNA co-transfection.

jetPRIME™ is a new versatile and powerful DNA and siRNA transfection reagent for day-to-day experiments.
jetPRIME™ ensures high DNA transfection efficiency and excellent gene silencing in a variety of adherent cells. Hence, jetPRIME™ is ideal for DNA/siRNA co-transfection. jetPRIME™ is very gentle to cells since it requires low amounts of nucleic acid and reagent during transfection.

1.5 ml is sufficient to perform ca. 375 transfections in 6-well plates.

Outstanding DNA transfection efficiency    
 
Using jetPRIME™ reagent, DNA transfection efficiencies range between 70 and 90% on adherent cell lines, using only 2 µg of DNA and 4 µl of reagent per well in 6-well plates (Fig. 1-2).


Fig. 1. Transfection efficiency assessed by FACS analysis in various cell lines 24h following transfection of 2 µg pCMV-GFP plasmid and 4 µl jetPRIME™ per well in 6-well plates.


Fig. 2. GFP expression in HEK-293 and SK-OV-3 cells 24 h after transfection using 2 µg pCMV-GFP plasmid and 4 µl of jetPRIME™ in
6-well plates.

Using jetPRIME™ reagent and DNA, transfection efficiencies range between 70 and 90 % on standard cell lines.
 

Economical: less DNA and less reagent needed   
 
jetPRIME™ is such a powerful in vitro transfection reagent that it only requires a small amount of plasmid DNA and reagent (Fig. 3). As a result, it is a very economical reagent.


Fig. 3. Amounts of DNA and reagent (jetPRIME™ and competitor) added per well for transfection according to manufacturers’ recommendations.

In addition to reducing costs, using less DNA also minimizes adverse cytotoxic effects triggered by transfection. Hence, jetPRIME™ is the reagent of choice for high transfection efficiency with excellent cell viability.
 

Better cell viability   
 

jetPRIME™ is extremely gentle to cells during transfection leading to increased cell viability (Fig. 4) and improved transfection results. Cells transfected with jetPRIME™ are healthy, while major cytotoxicity is observed with competitor.


Fig. 4. Phase contrast microscopy of HeLa cells 24 h after transfections performed according to the manufacturer’s recommendations for each reagent.

Excellent gene silencing    
 

jetPRIME™ leads to over 90% knockdown of endogenous gene expression in a variety of cell lines. For example, jetPRIME™-mediated transfection of HeLa cells with10 nM siRNA duplexes targeting endogenous lamin A/C in HeLa cells drastically reduces lamin A/C gene expression to barely detectable level (Fig. 5).


Fig. 5. Endogenous lamin A/C silencing using jetPRIME™. HeLa cells were transfected with 10 nM of 21-mer lamin A/C siRNA. After 48 h, lamin A/C silencing was assessed by immunofluorescence microscopy using an antibody against lamin A/C.

Co-transfection of DNA & siRNA   
 
jetPRIME™ is well suited for DNA and siRNA co-transfection experiments. It shows highly efficient gene silencing in a variety of cell lines with very low toxicity. Over 90% silencing is achieved in adherent cells, using 10 nM siRNA (Fig. 6).

   
Fig. 6. Exogenous luciferase gene silencing in several cell lines after DNA & siRNA co-transfection using jetPRIME™ performed with 400 ng pCMV-Luc and 10 nM of luciferase siRNA per well in 6-well plates.

Convenient protocol   
 

jetPRIME™ is an easy-to-use transfection reagent
(Fig. 7):

• Fast and easy to scale up and down

• Compatible with serum and antibiotics


Fig. 7. jetPRIME™ convenient protocol for DNA, siRNA and co-transfection of DNA and siRNA.