Multiplex Immunoassays
Save Time and Precious Samples by Performing Multiplex Immunoassays Using Standard Dual-laser Flow Cytometers
MultiBead assay technology utilizes the ability of commercially available flow cytometers to separate latex beads covalently coupled to antibodies on the basis of size and fluorescent intensity. MultiBead assays can simultaneously quantify up to 22 analytes in a single well by utilizing two sizes of beads (4.0 and 5.4 µm) and 11 different bead intensities. Each bead size is sub-fractioned into different populations based on their inherent fluorescent intensity by a 670 nm filter, while the analytes of interest are simultaneously quantified by a 575 nm filter that measures fluorescence associated with phycoerythrin (PE) conjugated detection antibody or analyte.
MultiBead assays are easy to perform and utilize a proprietary conjugation chemistry to covalently and efficiently couple the capture antibody to the bead of interest. This improves assay sensitivity when compared to beads that are coupled using traditional carboxy-conjugation methods. In addition, unlike traditional immunoassays, MultiBead assays can be used to simultaneously detect both large (immunometric assays) and small (competitive immunoassays) analytes from a single sample (See picture). To ensure accurate results, rigorous validation experiments are performed to make certain that each bead specifically detects the analyte of interest with no interference from other antibody-bead populations or sample components.
Simplify Data Analysis
The MultiBead Analysis Software is available free of charge with the purchase of any MultiBead kit to simplify and streamline data analysis. This Assay Designs exclusive software allows users to generate standard curves and determine sample concentrations for both immunometric and competitive assays in a mutiplex bead format using FCS or LMD files.
980-001, Inflammation 8-Plex Panel (Multiplex of the below) 1x96 well
985-002, IFN-gamma Singleplex Kit 1x96 well
985-003, IL-1ß Singleplex Kit 1x96 well
985-006, IL-4 Singleplex Kit 1x96 well
985-005, IL-6 Singleplex Kit 1x96 well
985-004, IL-8 Singleplex Kit 1x96 well
985-008, PGE2 Singleplex Kit 1x96 well
985-001, TNF-alfa Singleplex Kit 1x96 well
985-007, TXB2 Singleplex Kit 1x96 well
987-001, Extracellular Buffer Pack 1 (to be used with Singleplex) 1x96 well
